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Perforin 1 Antikörper (AA 413-552)

Der Maus Monoklonal Anti-Perforin 1-Antikörper wurde für IHC, ELISA, Coat und StM validiert. Er ist geeignet, Perforin 1 in Proben von Human zu detektieren.
Produktnummer ABIN6940388

Kurzübersicht für Perforin 1 Antikörper (AA 413-552) (ABIN6940388)

Target

Alle Perforin 1 (PRF1) Antikörper anzeigen
Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reaktivität

  • 92
  • 29
  • 9
  • 8
  • 6
  • 5
  • 2
  • 1
  • 1
  • 1
Human

Wirt

  • 65
  • 51
  • 3
Maus

Klonalität

  • 66
  • 53
Monoklonal

Konjugat

  • 65
  • 15
  • 14
  • 5
  • 5
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Perforin 1 Antikörper ist unkonjugiert

Applikation

  • 59
  • 46
  • 38
  • 24
  • 23
  • 23
  • 10
  • 7
  • 5
  • 5
  • 4
  • 4
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Immunohistochemistry (IHC), ELISA, Coating (Coat), Staining Methods (StM)

Klon

PRF1-2470
  • Bindungsspezifität

    • 8
    • 8
    • 6
    • 5
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 413-552

    Aufreinigung

    Purified by Protein A/G

    Immunogen

    Recombinant human Perforin-1 protein fragment (around aa 413-552) (exact sequence is proprietary)

    Isotyp

    IgG2c kappa
  • Applikationshinweise

    Positive Control: HDLM-2 cells. Human spleen tissue (IHC).

    Known Application: ELISA (For coating, order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Haltbarkeit

    24 months
  • Target

    Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

    Andere Bezeichnung

    PRF1

    Hintergrund

    Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.

    Molekulargewicht

    75kDa

    Gen-ID

    5551

    UniProt

    P14222

    Pathways

    Apoptose, Caspase Kaskade in der Apoptose
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