NEFL Antikörper

Produktnummer ABIN6940187

Dieses Maus Monoklonal-Antikörper erkennt spezifisch NEFL in IHC, IF, FACS und StM. Er zeigt eine Reaktivität gegenüber Human, Ratte, Schwein, Rind (Kuh) und Huhn.

Kurzübersicht für NEFL Antikörper (ABIN6940187)

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Reaktivität: Human, Ratte, Schwein, Rind (Kuh), Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NEFL Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Staining Methods (StM)

Klon: NR-4

Produktdetails anti-NEFL Antikörper

Spezifität: This MAb reacts with a 68 kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68 kDa (NF-L), 160 kDa (NF-M) and 200 kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.

Aufreinigung: Purified by Protein A/G

Immunogen: Crude neurofilament preparation from porcine spinal cord

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Positive Control: HEK293 cells, Brain, Neuroblastoma.

Known Application: Immunofluorescence (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu NEFL

Target: NEFL (Neurofilament, Light Polypeptide (NEFL))

Andere Bezeichnung: NEFL

Molekulargewicht: 68kDa

Gen-ID: 4747

UniProt: P07196