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MLH1 Antikörper

This anti-MLH1 antibody (ABIN6940057) is a Mouse Monoclonal antibody detecting MLH1 in ELISA, Coat. Suitable for Human.
Produktnummer ABIN6940057

Kurzübersicht für MLH1 Antikörper (ABIN6940057)

Target

Alle MLH1 Antikörper anzeigen
MLH1 (MutL Homolog 1 (MLH1))

Reaktivität

  • 89
  • 21
  • 20
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Human

Wirt

  • 73
  • 17
Maus

Klonalität

  • 67
  • 23
Monoklonal

Konjugat

  • 60
  • 4
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser MLH1 Antikörper ist unkonjugiert

Applikation

  • 54
  • 33
  • 21
  • 15
  • 15
  • 14
  • 14
  • 13
  • 13
  • 8
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
ELISA, Coating (Coat)

Klon

MLH1-1324
  • Spezifität

    This MAb recognizes a protein of 83 kDa, identified as MLH1. Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2).Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process, which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma, which plays a role in meiosis.

    Aufreinigung

    Purified by Protein A/G

    Immunogen

    Recombinant human MLH1 protein

    Isotyp

    IgG2b kappa
  • Applikationshinweise

    Positive Control: Molt-4, HeLa or Jurkat cells. Lymph node or Colon, breast, lung, testis, prostate or thyroid carcinoma.

    Known Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Haltbarkeit

    24 months
  • Target

    MLH1 (MutL Homolog 1 (MLH1))

    Andere Bezeichnung

    MLH1

    Molekulargewicht

    85kDa

    Gen-ID

    4292

    UniProt

    P40692

    Pathways

    DNA Reparatur, Production of Molecular Mediator of Immune Response
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