Rekombinanter Cytokeratin 18 Antikörper
Kurzübersicht für Rekombinanter Cytokeratin 18 Antikörper (ABIN6939939)
Target
Alle Cytokeratin 18 (KRT18) Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Spezifität
- This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
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Keine Kreuzreaktivität
- Huhn, Xenopus laevis
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Aufreinigung
- Purified by Protein A/G
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Immunogen
- Recombinant human full-length KRT18 protein
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Isotyp
- IgG
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Applikationshinweise
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Positive Control: HCT116, MCF-7, HeLa cells, Breast Cancer.
Known Application: Flow Cytometry (0.5-1 μg/million cells),Western Blot (0.5-2 μg/mL),Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 200 μg/mL
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Buffer
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C,-80 °C
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Informationen zur Lagerung
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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Haltbarkeit
- 24 months
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- Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))
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Andere Bezeichnung
- KRT18
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Molekulargewicht
- 45kDa
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Gen-ID
- 3875
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UniProt
- P05783
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Pathways
- Apoptose, Caspase Kaskade in der Apoptose
Target
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