IgD Antikörper

Produktnummer ABIN6939707

Der Maus Monoklonal anti-IgD Antikörper wird verwendet zum Nachweis von IgD in Proben von Human. Er wurde validiert für FACS, IHC und IF.

Kurzübersicht für IgD Antikörper (ABIN6939707)

Target: IgD

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IgD Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: IgD26

Produktdetails anti-IgD Antikörper

Verwendungszweck: IgD (Immunoglobulin Delta Heavy Chain) (B-Cell Marker) Antibody

Spezifität: Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through interchain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin D (IgD) exists as a monomer with light chains. It plays a biological role as a transmembrane receptor molecule, co-expressed with IgM on the surface of mature/naive B cells. In particular, it is found on spleen B cell surfaces. Compared to IgM, IgD exists in much lower numbers and is not expressed on immature B cells. IgD surface expression on B cells is regulated in part by IL-27. In mice, the inhibition of this immunoglobulin isotype does not cause a significant change to the immune system.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Full-length human IGHD protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Human tonsil lysate, NAMALWA cell lysate, Human tonsil and lymphoid tissues.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu IgD

Target: IgD

Andere Bezeichnung: IGHD

Substanzklasse: Antibody

Hintergrund:

Ig delta chain C region, IGHD, Immunoglobulin heavy constant delta

Human tonsil lysate, NAMALWA cell lysate, Human tonsil and lymphoid tissues.

Molekulargewicht: 44-80kDa (heavy chain), 21-25kDa (kappa light chain)

Gen-ID: 3495, 510635

UniProt: P01880