Rekombinanter HLA DQ Antikörper

Produktnummer ABIN6939634

Der Kaninchen Monoklonal anti-HLA DQ Antikörper (Klon HLA-DQA1-2866R) (ABIN6939634) detektiert spezifisch HLA DQ in FACS, IF und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Schwein.

Kurzübersicht für Rekombinanter HLA DQ Antikörper (ABIN6939634)

Target: HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Schwein

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser HLA DQ Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Klon: HLA-DQA1-2866R

Produktdetails anti-HLA DQ Antikörper

Verwendungszweck: Recombinant HLA-DQ (MHC II) Antibody

Spezifität: Recognizes a DQ antigen, which is a dimer of 60 kDa. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons, exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.This MAb strongly blocks cytotoxicity activity of T4-positive cytotoxic T cell clones.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human HLA-DQ protein

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Raji cells. Tonsil or lymph node.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Frozen only) (0.5-1 μg/mL for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu HLA DQ

Target: HLA DQ (HLA-DQ) (MHC Class II HLA-DQ (HLA-DQ))

Andere Bezeichnung: HLA-DQ

Hintergrund:

CELIAC1, DC 4 alpha chain Gluten sensitive enteropathy (celiac disease) (GSE), HLA class II histocompatibility antigen DQ(1) alpha chain, HLA class II histocompatibility antigen DQ(3) alpha chain, HLA DQA1, HLA DQB1, IDDM1, Leucocyte antigen DQA1, Major histocompatibility complex class II DQ alpha 1, Major histocompatibility complex class II DQ beta 1

Raji cells. Tonsil or lymph node.

Molekulargewicht: 60kDa

Gen-ID: 3117, 3118, 3119

UniProt: P01908, P01909, P01920