HLA-A Antikörper

Produktnummer ABIN6939620

Der Maus Monoklonal anti-HLA-A Antikörper wird verwendet zum Nachweis von HLA-A in Proben von Human. Er wurde validiert für FACS, IF und IHC.

Kurzübersicht für HLA-A Antikörper (ABIN6939620)

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HLA-A Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Klon: CATA-1

Produktdetails anti-HLA-A Antikörper

Verwendungszweck: HLA-Aw32 & HLA-A25 (MHC I) Antibody

Spezifität: This MAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31, B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This MAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, DRw10

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: THP-1 and Jurkat cells. Tonsil or lymph node.

Known Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu HLA-A

Target: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Andere Bezeichnung: HLA-A

Hintergrund:

HLA-A, HLA class 1A, MHC class I HLA-A

THP-1 and Jurkat cells. Tonsil or lymph node.

Molekulargewicht: ~41kDa

Gen-ID: 3105, 181244, 654404

UniProt: P01889

Pathways: T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response