Rekombinanter GFAP Antikörper

Produktnummer ABIN6939508

Dieses Maus Monoklonal-Antikörper erkennt spezifisch GFAP in WB, IHC, FACS und StM. Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Schwein, Rind (Kuh), Huhn und Kaninchen.

Kurzübersicht für Rekombinanter GFAP Antikörper (ABIN6939508)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Maus, Schwein, Rind (Kuh), Huhn, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GFAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Staining Methods (StM)

Klon: RASTRO-789

Produktdetails anti-GFAP Antikörper

Spezifität: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Aufreinigung: Purified by Protein A/G

Immunogen: Recombinant full-length human GFAP protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).

Known Application: Flow Cytometry (1-2 μg/million cells),Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP

Molekulargewicht: ~50kDa

Gen-ID: 2670

UniProt: P14136