FAT Atypical Cadherin 2 (FAT2) Antikörper

Produktnummer ABIN6939371

Dieses Anti--Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von in IF, FACS, IHC und StM. Geeignet für Human.

Kurzübersicht für FAT Atypical Cadherin 2 (FAT2) Antikörper (ABIN6939371)

Target: FAT Atypical Cadherin 2 (FAT2)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Staining Methods (StM)

Klon: 8C5

Produktdetails

Spezifität: Recognizes a protein of 480 kDa, which is identified as FAT2. The cadherins represent a family of Ca2+-dependent adhesion molecules that function to mediate cell-to-cell binding that is critical for the maintenance of structure and morphogenesis. Cadherins each contain a large extracellular domain at the N-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short C-terminal intracellular domain interacts with a variety of cytoplasmic proteins, including -catenin, to regulate cadherin function. The cadherin superfamily includes cadherins, protocadherins, desmogleins and desmocollins. FAT2 (FAT tumor suppressor homolog 2) is a single-pass type I membrane protein that belongs to the protocadherin subfamily of cadherins. FAT2 contains one Laminin G-like domain, two EGF-like domains and 32 cadherin domains and is believed to function as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development.

Aufreinigung: Purified by Protein A/G

Immunogen: Purified recombinant human FAT2 fusion protein.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Esophagus, Cervix, Uterus, Tonsil or Cerebellum.

Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Antigendetails

Target: FAT Atypical Cadherin 2 (FAT2)

Andere Bezeichnung: FAT2

Molekulargewicht: 480kDa

Gen-ID: 2196

UniProt: Q9NYQ8