FAT Atypical Cadherin 2 (FAT2) Antikörper (Biotin)

Produktnummer ABIN6155420

Produktdetails

Target: FAT Atypical Cadherin 2 (FAT2)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Verwendungszweck: Mouse Monoclonal anti-Protocadherin-FAT2 (8C5), Biotin Conjugate

Produktmerkmale: This antibody recognizes a protein of 480 kDa, which is identified as FAT2. The cadherins represent a family of Ca2 -dependent adhesion molecules that function to mediate cell-to-cell binding that is critical for the maintenance of structure and morphogenesis. Cadherins each contain a large extracellular domain at the N-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short C-terminal intracellular domain interacts with a variety of cytoplasmic proteins, including ?-catenin, to regulate cadherin function. The cadherin superfamily includes cadherins, protocadherins, desmogleins and desmocollins. FAT2 (FAT tumor suppressor homolog 2) is a single-pass type I membrane protein that belongs to the protocadherin subfamily of cadherins. FAT2 contains one Laminin G-like domain, two EGF-like domains and 32 cadherin domains and is believed to function as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified recombinant human FAT2 fusion protein

Klon: 8C5

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Esophagus, Cervix, Uterus, Tonsil or Cerebellum.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Antigendetails

Target: FAT Atypical Cadherin 2 (FAT2)

Andere Bezeichnung: Protocadherin-FAT2 (FAT2 Produkte)

Molekulargewicht: 480 kDa

Gen-ID: 2196, 591255

UniProt: Q9NYQ8