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Thymidine Phosphorylase Antikörper

Der Maus Monoklonal Anti-Thymidine Phosphorylase-Antikörper wurde für WB, IHC, IP und StM validiert. Er ist geeignet, Thymidine Phosphorylase in Proben von Human, Ratte und Maus zu detektieren.
Produktnummer ABIN6939276

Kurzübersicht für Thymidine Phosphorylase Antikörper (ABIN6939276)

Target

Alle Thymidine Phosphorylase (TYMP) Antikörper anzeigen
Thymidine Phosphorylase (TYMP)

Reaktivität

  • 71
  • 31
  • 24
  • 1
  • 1
  • 1
Human, Ratte, Maus

Wirt

  • 65
  • 16
  • 1
Maus

Klonalität

  • 64
  • 18
Monoklonal

Konjugat

  • 38
  • 9
  • 7
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Thymidine Phosphorylase Antikörper ist unkonjugiert

Applikation

  • 68
  • 36
  • 20
  • 19
  • 13
  • 13
  • 12
  • 9
  • 5
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Staining Methods (StM)

Klon

P-GF-44C
  • Spezifität

    Recognizes a protein (amino acid 482) of 55 kDa (in vivo 110 kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5'-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

    Aufreinigung

    Purified by Protein A/G

    Immunogen

    Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein

    Isotyp

    IgG1 kappa
  • Applikationshinweise

    Positive Control: HUVEC cells. Breast, Bladder, Lung or Kaposi tumors.

    Known Application: Western Blot (0.5-1 μg/mL), Immunoprecipitation (0.5-1 μg/500 μg protein lysate), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    200 μg/mL

    Buffer

    10 mM PBS with 0.05 % BSA & 0.05 % azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Lagerung

    4 °C,-80 °C

    Informationen zur Lagerung

    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

    Haltbarkeit

    24 months
  • Target

    Thymidine Phosphorylase (TYMP)

    Andere Bezeichnung

    TYMP

    Molekulargewicht

    55kDa

    Gen-ID

    1890

    UniProt

    P19971

    Pathways

    Signaling Events mediated by VEGFR1 and VEGFR2
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