CFTR Antikörper (AA 258-385)

Produktnummer ABIN6939092

Produktdetails anti-CFTR Antikörper

Target: CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))

Bindungsspezifität: AA 258-385

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CFTR Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS)

Spezifität: Recognizes a protein of 165-170kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

Kreuzreaktivität (Details): Human,

Aufreinigung: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 258-385) of human CFTRprotein (exact sequence is proprietary)

Klon: CFTR-1643

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

Positive Control: MOLT-4 cells. Human pancreas, kidney or placenta.

Known Application: Flow Cytometry (1-2 μg/million cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Haltbarkeit: 24 months

Details zu CFTR

Target: CFTR (Cystic Fibrosis Transmembrane Conductance Regulator (ATP-Binding Cassette Sub-Family C, Member 7) (CFTR))

Andere Bezeichnung: CFTR (CFTR Produkte)

Hintergrund: ABC35, ATP Binding Cassette Superfamily C Member 7 (ABCC7), cAMP-dependent chloride channel, CFTR/MRP, Channel conductance-controlling ATPase, Cystic Fibrosis Transmembrane Conductance Regulator, MRP7, TNR CFTR,CFTR (Cystic Fibrosis Transmembrane Conductance Regulator)
Cellular localisation: Cell surface. Cytoplasm.

Molekulargewicht: 165-170kDa

Gen-ID: 1080, 489786, 621460

UniProt: P13569