Thymidine Phosphorylase Antikörper
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- Target Alle Thymidine Phosphorylase (TYMP) Antikörper anzeigen
- Thymidine Phosphorylase (TYMP)
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Thymidine Phosphorylase Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- Recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5'-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.
- Kreuzreaktivität (Details)
- Human. Mouse. Rat.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein
- Klon
- P-GF-44C
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product TYMP Primärantikörper
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- Applikationshinweise
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Known_Application: Western Blot (1-2 μg/mL), Immunoprecipitation (0.5-1 μg/500 μg protein lysate), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HUVEC cells. Breast,Spleen, Bladder, Lung or Kaposi tumors.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
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- Target
- Thymidine Phosphorylase (TYMP)
- Andere Bezeichnung
- TYMP (TYMP Produkte)
- Synonyme
- ECGF antikoerper, ECGF1 antikoerper, MEDPS1 antikoerper, MNGIE antikoerper, MTDPS1 antikoerper, PDECGF antikoerper, TP antikoerper, hPD-ECGF antikoerper, 2900072D10Rik antikoerper, Ecgf1 antikoerper, PD-ECGF antikoerper, Pdgfec antikoerper, typh antikoerper, thymidine phosphorylase antikoerper, thymidine phosphorylase DeoA antikoerper, AMP phosphorylase antikoerper, Thymidine phosphorylase antikoerper, TYMP antikoerper, Tymp antikoerper, deoA antikoerper, Dd586_0614 antikoerper, Kvar_4413 antikoerper, NMAG_RS19330 antikoerper, Snov_1187 antikoerper, Fbal_2856 antikoerper, Ftrac_2071 antikoerper, Intca_1216 antikoerper, ARCVE_RS10415 antikoerper, Sphch_4174 antikoerper, Sinme_3461 antikoerper, METOK_RS06355 antikoerper, Mesop_1831 antikoerper, typh antikoerper
- Hintergrund
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ECGF, ECGF1, Gliostatin, hPD-ECGF, MEDPS1, MNGIE, MTDPS1, PD-ECGF, PDECGF, Platelet-derived endothelial cell growth factor, TdRPase, Thymidine phosphorylase, TP, Tymp,Thymidine Phosphorylase / PD-ECGF (Angiogenesis Marker)
Cellular localisation: Cytoplasmic and Nuclear - Molekulargewicht
- 55kDa
- Gen-ID
- 1890, 180903
- UniProt
- P19971
- Pathways
- Signaling Events mediated by VEGFR1 and VEGFR2
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