TCF4 Antikörper (AA 365-671)

Produktnummer ABIN6938516

Dieses Maus Monoklonal-Antikörper erkennt spezifisch TCF4 in WB, ELISA, Coat und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für TCF4 Antikörper (AA 365-671) (ABIN6938516)

Target: TCF4 (Transcription Factor 4 (TCF4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TCF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: TCF4-1705

Produktdetails anti-TCF4 Antikörper

Bindungsspezifität: AA 365-671

Spezifität: Recognizes a protein of 71kDa, identified as Transcription Factor 4 (TCF4). It is a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human TCF4 protein fragment (around aa 365-671) (exact sequence is proprietary)

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), ,Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or HepG2 cells. Human bladder, colon or tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu TCF4

Target: TCF4 (Transcription Factor 4 (TCF4))

Andere Bezeichnung: TCF4

Hintergrund: BHLHb19, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, ITF-2, PTHS, SEF2, SL3-3 enhancer factor 2, TCF4, Transcription factor 4,TCF4 (Transcription Factor 4)
Cellular localisation: Nucleus. Cytoplasm.

Molekulargewicht: 60kDa

Gen-ID: 6925, 605153

UniProt: P15884

Pathways: WNT Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus