RAD51 Antikörper (AA 1-134)

Produktnummer ABIN6938307

Der Maus Monoklonal Anti-RAD51-Antikörper wurde für FACS und IHC (f) validiert. Er ist geeignet, RAD51 in Proben von Human zu detektieren.

Kurzübersicht für RAD51 Antikörper (AA 1-134) (ABIN6938307)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RAD51 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: RAD51-2702

Produktdetails anti-RAD51 Antikörper

Bindungsspezifität: AA 1-134

Spezifität: RAD51 is one of the key factors of DNA repair by homologous recombination and has been shown to have anti-apoptotic activity in tumor cells. RAD51 protein interacts with a variety of tumor suppressor proteins including p53, BRCA1 and BRCA2. Elevated expression of RAD51 enhances radio-resistance of human tumor cells. Overexpression of RAD51 protein in tumor cells renders them resistant against cytotoxic drugs like Cisplatin. RAD51 interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of RAD51. High-level expression of RAD51 has been observed in a variety of human malignancies.RAD51 overexpression correlates with histological grading of the tumor in invasive ductal mammary carcinoma.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human RAD51 protein (around aa 1-134) (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: K562, HeLa, MCF-7 cells. Colon or breast Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Andere Bezeichnung: RAD51

Hintergrund: RCA1/BRCA2 containing complex, subunit 5, BRCC5, DNA repair protein RAD51, FANCR, HsT16930, MRMV2, RAD51, RAD51 recombinase, RAD51A, RECA, RecA like protein, Recombination protein A,RAD51 (Prognostic and Response to Chemotherapy Marker)
Cellular localisation: Nuclear

Molekulargewicht: 37kDa

Gen-ID: 5888, 631709

UniProt: Q06609

Pathways: DNA Reparatur