Perforin 1 Antikörper (AA 413-552)

Produktnummer ABIN6938235

Der Maus Monoklonal Anti-Perforin 1-Antikörper wurde für IHC, ELISA, Coat und IHC (f) validiert. Er ist geeignet, Perforin 1 in Proben von Human zu detektieren.

Kurzübersicht für Perforin 1 Antikörper (AA 413-552) (ABIN6938235)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Perforin 1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: PRF1-2470

Produktdetails anti-Perforin 1 Antikörper

Bindungsspezifität: AA 413-552

Spezifität: Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human Perforin-1 protein fragment (around aa 413-552) (exact sequence is proprietary)

Isotyp: IgG2c kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HDLM-2 cells. Human spleen tissue (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Andere Bezeichnung: PRF1

Hintergrund: Cytolysin, FLH2, HPLH2, Lymphocyte pore-forming protein, PRF1 (pore forming protein 1), Perforin-1, PFP, PGFL, PIGF, PIGF-2, PLGF,Perforin-1 (Pore Forming Protein) (Apoptosis Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 75kDa

Gen-ID: 5551, 2200

UniProt: P14222

Pathways: Apoptose, Caspase Kaskade in der Apoptose