MSH2 Antikörper (AA 327-427)

Produktnummer ABIN6937901

Dieser Maus Monoklonal Antikörper detektiert spezifisch MSH2 in WB, IHC, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte, Hund, Meerschweinchen und Hamster.

Kurzübersicht für MSH2 Antikörper (AA 327-427) (ABIN6937901)

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Reaktivität: Human, Maus, Ratte, Hund, Meerschweinchen, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MSH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: MSH2-2622

Produktdetails anti-MSH2 Antikörper

Bindungsspezifität: AA 327-427

Verwendungszweck: MSH2 (DNA Mismatch Repair Marker) Antibody

Spezifität: Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC). Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45 % of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.

Kreuzreaktivität (Details): Human. Dog

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 327-427) of human MSH2 protein (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: A549 or HepG2 cells. Colon, Thyroid, Testis or Lymph Node.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MSH2

Target: MSH2 (Mismatch Repair Protein 2 (MSH2))

Andere Bezeichnung: MSH2

Hintergrund:

BAT26, COCA1, DNA mismatch repair protein Msh2, FCC1, hMSH2, HNPCC1, LCFS2, MSH2, MutS homolog 2, MutS homolog 2 colon cancer nonpolyposis type 1, MutS protein homolog 2

A549 or HepG2 cells. Colon, Testis or Lymph Node., Thyroid, Human Testis, Mouse Testis, Rat Testis, Hamster Testis, Guinea pig Testis, K-562 or HeLa

Molekulargewicht: 100kDa

Gen-ID: 4436, 597656

Pathways: DNA Reparatur, Production of Molecular Mediator of Immune Response