Rekombinanter Cytokeratin 2 Antikörper

Produktnummer ABIN6937734

Der Kaninchen Monoklonal anti-Cytokeratin 2 Antikörper wird verwendet zum Nachweis von Cytokeratin 2 in Proben von Human, Maus und Ratte. Er wurde validiert für WB, IHC, IF und FACS.

Kurzübersicht für Rekombinanter Cytokeratin 2 Antikörper (ABIN6937734)

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: KRTH-2147R

Produktdetails anti-Cytokeratin 2 Antikörper

Verwendungszweck: Recombinant Cytokeratin, Basic (Type II or HMW) Antibody

Spezifität: This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

Kreuzreaktivität (Details): Human. Mouse. Rat.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Solubilized keratin extract from human stratum corneum

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: MCF-7, PC12 cells, Skin, Prostate carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Cytokeratin 2

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Andere Bezeichnung: KRT76

Hintergrund:

KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9

MCF-7, PC12 cells, Prostate carcinoma., Skin

Molekulargewicht: 50-67kDa

Gen-ID: 51350, 654392

UniProt: Q01546