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Rekombinanter Cytokeratin 2 Antikörper

Der Kaninchen Monoklonal Anti-Cytokeratin 2-Antikörper wurde für WB, IF, FACS und IHC (f) validiert. Er ist geeignet, Cytokeratin 2 in Proben von Human zu detektieren.
Produktnummer ABIN6937734

Kurzübersicht für Rekombinanter Cytokeratin 2 Antikörper (ABIN6937734)

Target

Alle Cytokeratin 2 (KRT76) Antikörper anzeigen
Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Antikörpertyp

Recombinant Antibody

Reaktivität

  • 34
  • 13
  • 8
  • 1
  • 1
Human

Wirt

  • 22
  • 11
  • 1
Kaninchen

Klonalität

  • 21
  • 13
Monoklonal

Konjugat

  • 24
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser Cytokeratin 2 Antikörper ist unkonjugiert

Applikation

  • 18
  • 12
  • 10
  • 7
  • 6
  • 5
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon

KRTH-2147R
  • Spezifität

    This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.

    Kreuzreaktivität (Details)

    Human. Mouse. Rat.

    Aufreinigung

    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

    Immunogen

    Solubilized keratin extract from human stratum corneum

    Isotyp

    IgG
  • Applikationshinweise

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: MCF-7, PC12 cells, Skin, Prostate carcinoma.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Konzentration

    1.0 mg/mL

    Buffer

    Prepared in 10 mM PBS, WITHOUT BSA and Azide.

    Konservierungsmittel

    Azide free

    Lagerung

    -20 °C,-80 °C

    Informationen zur Lagerung

    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

    Haltbarkeit

    24 months
  • Target

    Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

    Andere Bezeichnung

    KRT76

    Hintergrund

    KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9,Cytokeratin, Basic (Type II or HMW)
    Cellular localisation: Cytoplasmic

    Molekulargewicht

    50-67kDa

    Gen-ID

    51350, 654392

    UniProt

    Q01546
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