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Rekombinanter Cytokeratin 2 Antikörper

KRT76 Reaktivität: Human WB, IF, FACS, IHC (f) Wirt: Kaninchen Monoclonal KRTH-2147R unconjugated Recombinant Antibody
Produktnummer ABIN6937734
  • Target Alle Cytokeratin 2 (KRT76) Antikörper anzeigen
    Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))
    Antikörpertyp
    Recombinant Antibody
    Reaktivität
    • 32
    • 13
    • 8
    • 1
    • 1
    Human
    Wirt
    • 20
    • 11
    • 1
    Kaninchen
    Klonalität
    • 19
    • 13
    Monoklonal
    Konjugat
    • 22
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Cytokeratin 2 Antikörper ist unkonjugiert
    Applikation
    • 16
    • 12
    • 10
    • 6
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Spezifität
    This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
    Kreuzreaktivität (Details)
    Human. Mouse. Rat.
    Aufreinigung
    1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
    Immunogen
    Solubilized keratin extract from human stratum corneum
    Klon
    KRTH-2147R
    Isotyp
    IgG
    Top Product
    Discover our top product KRT76 Primärantikörper
  • Applikationshinweise

    Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

    Positive_Control: MCF-7, PC12 cells, Skin, Prostate carcinoma.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL
    Buffer
    Prepared in 10 mM PBS, WITHOUT BSA and Azide.
    Konservierungsmittel
    Azide free
    Lagerung
    -20 °C,-80 °C
    Informationen zur Lagerung
    Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
    Haltbarkeit
    24 months
  • Target
    Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))
    Andere Bezeichnung
    KRT76 (KRT76 Produkte)
    Hintergrund
    KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9,Cytokeratin, Basic (Type II or HMW)
    Cellular localisation: Cytoplasmic
    Molekulargewicht
    50-67kDa
    Gen-ID
    51350, 654392
    UniProt
    Q01546
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