Rekombinanter Cytokeratin 2 Antikörper
Kurzübersicht für Rekombinanter Cytokeratin 2 Antikörper (ABIN6937734)
Target
Alle Cytokeratin 2 (KRT76) Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Spezifität
- This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands, there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
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Kreuzreaktivität (Details)
- Human. Mouse. Rat.
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Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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Immunogen
- Solubilized keratin extract from human stratum corneum
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Isotyp
- IgG
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Applikationshinweise
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: MCF-7, PC12 cells, Skin, Prostate carcinoma.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1.0 mg/mL
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Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
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Konservierungsmittel
- Azide free
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Lagerung
- -20 °C,-80 °C
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Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
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Haltbarkeit
- 24 months
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- Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))
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Andere Bezeichnung
- KRT76
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Hintergrund
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KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9,Cytokeratin, Basic (Type II or HMW)
Cellular localisation: Cytoplasmic -
Molekulargewicht
- 50-67kDa
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Gen-ID
- 51350, 654392
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UniProt
- Q01546
Target
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