Cytokeratin 2 Antikörper

Produktnummer ABIN6937731

Der Maus Monoklonal anti-Cytokeratin 2 Antikörper wird verwendet zum Nachweis von Cytokeratin 2 in Proben von Human. Er wurde validiert für WB, IHC, IF und FACS.

Kurzübersicht für Cytokeratin 2 Antikörper (ABIN6937731)

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cytokeratin 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: DE-SQ

Produktdetails anti-Cytokeratin 2 Antikörper

Verwendungszweck: Cytokeratin, Basic (Type II or HMW) Antibody

Spezifität: This antibody is specific to cytokeratin 13, 14, 15 and 16. This antibody stains squamous carcinomas and adeno-squamous carcinomas but does not stain adenocarcinoma. This antibody is useful in differential staining of squamous carcinomas from adenocarcinomas and to differentiate benign and malignant tumors of prostate glands.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Cytoskeletal fraction prepared from human gingiva by detergent/salt extraction

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: PC12 cells. Tonsil, Skin, Prostate, Esophagus, Ectocervix or Squamous carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Cytokeratin 2

Target: Cytokeratin 2 (KRT76) (Cytokeratin-2P (KRT76))

Andere Bezeichnung: KRT76

Hintergrund:

KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9

Ectocervix or Squamous carcinoma., Esophagus, PC12 cells. Tonsil, prostate, Skin

Molekulargewicht: 50-67kDa

Gen-ID: 51350, 654392

UniProt: Q01546