Rekombinanter Insulin Receptor Antikörper (Extracellular Domain)

Produktnummer ABIN6937575

Der Kaninchen Monoklonal anti-Insulin Receptor Antikörper wird verwendet zum Nachweis von Insulin Receptor in Proben von Human. Er wurde validiert für IHC.

Kurzübersicht für Rekombinanter Insulin Receptor Antikörper (Extracellular Domain) (ABIN6937575)

Target: Insulin Receptor (INSR)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Insulin Receptor Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: INSR-2277R

Produktdetails anti-Insulin Receptor Antikörper

Bindungsspezifität: Extracellular Domain

Verwendungszweck: Recombinant Insulin Receptor Alpha Antibody

Spezifität: The insulin receptor (INSR) is a heterodimeric protein complex that has an intracellular subunit, which is disulfide-linked to a transmembrane segment. The insulin ligand binds to the INSR and initiates molecular signaling pathways that promote glucose uptake in cells and glycogen synthesis. Insulin binding to INSR induces phosphorylation of intra-cellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin. Type 1 diabetes is an autoimmune condition of the endocrine pancreas that results in destruction of insulin secreting cells and a progressive loss in insulin-sensitive glucose uptake by cells.

Kreuzreaktivität (Details): Human.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of extracellular domain of human Insulin Receptor alpha (exact sequence is proprietary)

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat cells. Human pancreas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Insulin Receptor

Target: Insulin Receptor (INSR)

Andere Bezeichnung: INSR

Hintergrund:

CD220, HHF5, HIR A, INSR, Insulin receptor, Insulin receptor subunit alpha, IR

Jurkat cells. Human pancreas.

Molekulargewicht: 135kDa

Gen-ID: 3643, 465744

UniProt: P06213

Pathways: NF-kappaB Signalweg, RTK Signalweg, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity