ICOSLG Antikörper (AA 23-149)

Produktnummer ABIN6937473

Der Maus Monoklonal Anti-ICOSLG-Antikörper wurde für WB, ELISA, IHC, FACS, IF, Coat und IHC (f) validiert. Er ist geeignet, ICOSLG in Proben von Human zu detektieren.

Kurzübersicht für ICOSLG Antikörper (AA 23-149) (ABIN6937473)

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ICOSLG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: ICOSL-3111

Produktdetails anti-ICOSLG Antikörper

Bindungsspezifität: AA 23-149

Spezifität: This MAb recognizes a protein of 36 kDa, identified as ICOS-L. It is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion. ICOS-L interactions play an essential role in T cell-dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes. ICOS-L protein is present in myeloid leukocytes, and by Northern blot there are 2.4, 3.0, and 7.0 kb transcripts in brain, heart, kidney, and liver, with lower expression in colon and thymus, and a 1.1 kb transcript in leukocytes. Tumor necrosis factor alpha (TNF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) enhance its expression. LPS-induced up-regulation of ICOS-L is dependent on the MyD88-dependent signaling pathway.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human ICOSLG (B7RP-1) protein (around aa 23-149) (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U937, HL60, HuT78 and HUVEC lysates (WB). Human tonsil tissue (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu ICOSLG

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Andere Bezeichnung: ICOSLG

Hintergrund: B7 homolog 2 (B7H2), B7 like protein Gl50, B7 related protein 1 (B7RP1), CD275, CD275 antigen, GL50, ICOS LG, ICOS ligand (ICOSL), Inducible T cell co stimulator ligand, LICOS, Transmembrane protein B7 H2 ICOS ligand,ICOS-L / ICOS Ligand / B7RP-1 (Immuno-Oncology Target)
Cellular localisation: Cell Surface and Cytoplasmic

Molekulargewicht: 36kDa

Gen-ID: 23308, 14155

UniProt: O75144

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation