ICOSLG Antikörper (AA 23-149)

Produktnummer ABIN6939407

Dieser Maus Monoklonal Antikörper detektiert spezifisch ICOSLG in IHC, ELISA, FACS und IF. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für ICOSLG Antikörper (AA 23-149) (ABIN6939407)

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ICOSLG Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: ICOSL-3111

Produktdetails anti-ICOSLG Antikörper

Bindungsspezifität: AA 23-149

Verwendungszweck: ICOS-L / ICOS Ligand / B7RP-1 (Immuno-Oncology Target) Antibody

Spezifität: This MAb recognizes a protein of 36 kDa, identified as ICOS-L. It is a ligand for the ICOS receptor that initiates T and B cell proliferation and cytokine secretion. ICOS-L interactions play an essential role in T cell-dependent B cell activation in peripheral lymphoid organs such as spleen and lymph nodes. ICOS-L protein is present in myeloid leukocytes, and by Northern blot there are 2.4, 3.0, and 7.0 kb transcripts in brain, heart, kidney, and liver, with lower expression in colon and thymus, and a 1.1 kb transcript in leukocytes. Tumor necrosis factor alpha (TNF), granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4) enhance its expression. LPS-induced up-regulation of ICOS-L is dependent on the MyD88-dependent signaling pathway.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human ICOSLG (B7RP-1) protein (around aa 23-149) (exact sequence is proprietary)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: U937, HL60, HuT78 and HUVEC lysates (WB). Human tonsil tissue (IHC).

Known Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 min at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu ICOSLG

Target: ICOSLG (Inducible T-Cell Co-Stimulator Ligand (ICOSLG))

Andere Bezeichnung: ICOSLG

Hintergrund:

B7 homolog 2 (B7H2), B7 like protein Gl50, B7 related protein 1 (B7RP1), CD275, CD275 antigen, GL50, ICOS LG, ICOS ligand (ICOSL), Inducible T cell co stimulator ligand, LICOS, Transmembrane protein B7 H2 ICOS ligand

HL60, HuT78 and HUVEC lysates (WB). Human tonsil tissue (IHC)., U937

Molekulargewicht: 36kDa

Gen-ID: 23308, 14155

UniProt: O75144

Pathways: Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation