GZMB Antikörper (AA 73-187)

Produktnummer ABIN6937361

Dieses Maus Monoklonal-Antikörper erkennt spezifisch GZMB in WB, IHC und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für GZMB Antikörper (AA 73-187) (ABIN6937361)

Target: GZMB (Granzyme B (GZMB))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GZMB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: GZMB-3014

Produktdetails anti-GZMB Antikörper

Bindungsspezifität: AA 73-187

Spezifität: Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin's Disease.

Kreuzreaktivität (Details): Human. Dog

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human GZMB protein (around aa 73-187) (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.

Positive_Control: A431 cells. Human tonsil, spleen or Hodgkin's Lymphoma tissue (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu GZMB

Target: GZMB (Granzyme B (GZMB))

Andere Bezeichnung: GZMB

Hintergrund: Cathepsin G-like 1, CCPI, CGL1, CSPB, CTLA-1, CTSGL1, Cytotoxic serine protease B, Cytotoxic T lymphocyte associated serine esterase 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, GRB, Human lymphocyte protein (Hlp), Lymphocyte protease, SECT, T-cell serine protease 1-3E,Granzyme B (NK/T-Cell Lymphoma Marker)
Cellular localisation: Cytoplasmic

Molekulargewicht: 29-32kDa

Gen-ID: 3002, 1051

UniProt: P10144

Pathways: Apoptose, Caspase Kaskade in der Apoptose