Rekombinanter GFAP Antikörper

Produktnummer ABIN6937313

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch GFAP in WB, IHC, IF und FACS. Es zeigt Reaktivität gegenüber Proben von Human, Ratte, Maus, Rind (Kuh), Schwein, Kaninchen, Huhn, Meerschweinchen, Hund und Hamster.

Kurzübersicht für Rekombinanter GFAP Antikörper (ABIN6937313)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Schwein, Kaninchen, Huhn, Meerschweinchen, Hund, Hamster

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser GFAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: ASTRO-1974R

Produktdetails anti-GFAP Antikörper

Verwendungszweck: Recombinant GFAP (Astrocyte & Neural Stem Cell Marker) Antibody

Spezifität: This MAb recognizes a protein of ~50 kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Kreuzreaktivität (Details): Human. Mouse. Rat. Cow. Pig. Rabbit. Chicken. Dog

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human full-length GFAP protein

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells),Immunofluorescence (2-3 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: T98G cells (FACS). Brain lysate (WB). Brain or Astrocytoma (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Andere Bezeichnung: GFAP

Hintergrund:

Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)

T98G cells (FACS). Human Brain, Mouse Brain, Rat Brain, Hamster Brain or Guinea pig Brain (WB). Brain or Astrocytoma (IHC).

Molekulargewicht: ~50kDa

Gen-ID: 2670, 514227

UniProt: P14136