FOXA1 Antikörper (AA 372-472)

Produktnummer ABIN6937255

Dieses Anti-FOXA1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von FOXA1 in WB, FACS, IF, ISt und IHC (f). Geeignet für Human.

Kurzübersicht für FOXA1 Antikörper (AA 372-472) (ABIN6937255)

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FOXA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: FOXA1-1514

Produktdetails anti-FOXA1 Antikörper

Bindungsspezifität: AA 372-472

Spezifität: The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (i± and i²), HNF-3 (i±, i² and i³), HNF-4 (i± and i³), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

Keine Kreuzreaktivität: Ratte (Rattus)

Kreuzreaktivität (Details): Human. Does not react with Rat. Others not known.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human FOXA1 protein fragment (aa372-472) (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (0.5-1 μg/mL), ,Western Blotting (0.5-1 μg/mL), ,Immunohistology (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HepG-2 or MCF-7 cells. Liver, Pancreas, Bladder, Prostate, Colon and Lung and Breast.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu FOXA1

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Andere Bezeichnung: FOXA1

Hintergrund: Forkhead box protein A1 (FOXA1), Hepatocyte nuclear factor 3-alpha (HNF-3-alpha or HNF-3A or HNF3A), Transcription factor 3A (TCF-3A or TCF3A),FOXA1 / HNF3A
Cellular localisation: Nuclear

Molekulargewicht: 49kDa

Gen-ID: 3169, 163484

UniProt: P55317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis