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FOXA1 Antikörper (C-Term)

Verified FOXA1 Reaktivität: Human, Maus WB, IF, FACS, ELISA Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN185021
  • Target Alle FOXA1 Antikörper anzeigen
    FOXA1 (Forkhead Box A1 (FOXA1))
    Bindungsspezifität
    • 30
    • 16
    • 12
    • 9
    • 9
    • 7
    • 6
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 132
    • 65
    • 38
    • 7
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Maus
    Wirt
    • 68
    • 64
    Ziege
    Klonalität
    • 77
    • 55
    Polyklonal
    Konjugat
    • 75
    • 8
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser FOXA1 Antikörper ist unkonjugiert
    Applikation
    • 87
    • 53
    • 52
    • 45
    • 38
    • 35
    • 24
    • 12
    • 6
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA
    Verwendungszweck
    FOXA1 / HNF3A
    Sequenz
    GVYSRPVLNT S
    Kreuzreaktivität
    Rind (Kuh), Human, Maus, Schwein, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence C-GVYSRPVLNTS, from the C Terminus of the protein sequence according to NP_004487.2.
    Isotyp
    IgG
  • Applikationshinweise

    Western Blot: Approx 50 kDa band observed in nuclear lysates of cell line HepG2 and approx 50-55 kDa in Mouse Liver and in nuclear lysates of cell line MCF7 calculated MW of 49.1 kDa according to Human NP_004487.2 and 48.9 kDa according to Mouse NP_032285.2)

    Peptide ELISA: antibody detection limit dilution 1:128000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 and U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10u

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Bjerke, Pietrzak, Melhuish, Frierson, Paschal, Wotton: "Prostate cancer induced by loss of Apc is restrained by TGFβ signaling." in: PLoS ONE, Vol. 9, Issue 3, pp. e92800, (2014) (PubMed).

  • Target
    FOXA1 (Forkhead Box A1 (FOXA1))
    Andere Bezeichnung
    FOXA1 (FOXA1 Produkte)
    Synonyme
    HNF3A antikoerper, TCF3A antikoerper, Hnf-3a antikoerper, Hnf3a antikoerper, Tcf-3a antikoerper, Tcf3a antikoerper, fkd7 antikoerper, forkhead-7 antikoerper, HNF3-alpha antikoerper, HNF3alpha antikoerper, fkh2 antikoerper, foxa1 antikoerper, hnf3a antikoerper, tFoxA1 antikoerper, tcf3a antikoerper, xfkh2 antikoerper, fkh antikoerper, FOXA1 antikoerper, Ci-fkh antikoerper, HNF-3a antikoerper, forkhead box A1 antikoerper, forkhead box A1 S homeolog antikoerper, forkhead homolog antikoerper, forkhead box A1 L homeolog antikoerper, FOXA1 antikoerper, Foxa1 antikoerper, foxa1 antikoerper, foxa1.S antikoerper, fkh antikoerper, foxa1.L antikoerper
    Hintergrund
    FOXA1, HNF3A, forkhead box A1, TCF3A, MGC33105, hepatocyte nuclear factor 3, alpha
    Gen-ID
    3169, 15375, 25098
    NCBI Accession
    NP_004487
    Pathways
    Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis
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