CD72 Antikörper
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- Target Alle CD72 Antikörper anzeigen
- CD72 (CD72 Molecule (CD72))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD72 Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- CD5 has been identified as a transmembrane glycoprotein that is expressed on 70 % of normal peripheral blood lymphocytes and on virtually all T lymphocytes in thymus and peripheral blood. Activation of T cells through the T cell receptor (TCR) results in tyrosine phosphorylation of CD5, and the absence of CD5 renders T cells hyper-responsive to TCR-mediated activation. CD5 associates with the TCR/CD3 chain, and with the Src family kinase, Lck p56. The C-type lectin superfamily member CD72 is a cell surface negative regulator of B cell activation from the pro-B through the mature B cell stage. CD72 serves as a receptor for CD5. The ability of lymphocytes to respond to antigenic or mitogenic stimulation utilizes both positive and negative regulatory proteins that influence the threshold for responsiveness. The human CD72 gene maps to chromosome 9p13.3 and encodes a transmembrane glycoprotein that contains an ITIM. Upon tyrosine phosphorylation, the CD72 ITIM recruits SH2-containing phosphatases such as SHP-1, resulting in downregulation of cell activation. CD72-/- mice contain hyperproliferative B cells.
- Kreuzreaktivität (Details)
- Human.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- HF B1 plasmacytoid cell line.
- Klon
- BU40
- Isotyp
- IgG2a kappa
- Top Product
- Discover our top product CD72 Primärantikörper
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- Applikationshinweise
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Known_Application: ELISA (For coating, order without BSA), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: K562 cel lysate. Tonsil or Lymph Node.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
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- Target
- CD72 (CD72 Molecule (CD72))
- Andere Bezeichnung
- CD72 (CD72 Produkte)
- Synonyme
- CD72c antikoerper, Ly-19 antikoerper, Ly-32 antikoerper, Ly-m19 antikoerper, Lyb-2 antikoerper, CD72b antikoerper, LYB2 antikoerper, CD72 antigen antikoerper, CD72 molecule antikoerper, Cd72 antikoerper, CD72 antikoerper
- Hintergrund
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B cell differentiation antigen CD72, CD72b, CD72c, Ly-19, Ly-32, Ly-m19, LYB2, Lymphocyte antigen 32,CD72 (B Cell Marker)
Cellular localisation: Membrane - Molekulargewicht
- 45kDa
- Gen-ID
- 971, 116481
- UniProt
- P21854
- Pathways
- BCR Signaling
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