CD40 Ligand Antikörper (AA 108-261)
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- Target Alle CD40 Ligand (CD40LG) Antikörper anzeigen
- CD40 Ligand (CD40LG)
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Bindungsspezifität
- AA 108-261
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD40 Ligand Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- CD40LG expression is mainly confined to the CD4-positive-T-cell subset. Its expression is induced shortly after T-cell activation and represents an early activation marker of T lymphocytes. CD40 is constitutively expressed mainly on B cells, macrophages, and dendritic cells. The CD40-CD40L pathway has been shown to play multiple functional roles in the healthy immune system. It enhances the antigen-specific T-cell response through the activation of dendritic cells and the induction of interleukin-12 production. For example, engagement of CD40 on endothelial cells by activated T cells expressing CD40L leads to upregulation of adhesion molecules such as ICAM-1, VCAM-1, and E-selectin. Activation of APC by CD40-CD40L interaction induces the production of inflammatory cytokines, chemokines, NO, and metalloproteinases. Interaction of CD4-positiveCD40LG-positiveT cells with CD40 on B cells leads to B-cell differentiation, proliferation, immunoglobulin (Ig) isotype switching, and formation of memory B cells.
- Kreuzreaktivität (Details)
- Human.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant fragment (around aa 108-261) of human CD40LG protein (exact sequence is proprietary)
- Klon
- CD40LG-2761
- Isotyp
- IgG2b kappa
- Top Product
- Discover our top product CD40LG Primärantikörper
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- Applikationshinweise
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Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: Jurkat cells. Tonsil, Spleen or Thymus.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
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- Target
- CD40 Ligand (CD40LG)
- Andere Bezeichnung
- CD40LG (CD40LG Produkte)
- Synonyme
- CD154 antikoerper, cd40l antikoerper, CD40L antikoerper, HIGM1 antikoerper, IGM antikoerper, IMD3 antikoerper, T-BAM antikoerper, TNFSF5 antikoerper, TRAP antikoerper, gp39 antikoerper, hCD40L antikoerper, CD40-L antikoerper, Cd40l antikoerper, Ly-62 antikoerper, Ly62 antikoerper, Tnfsf5 antikoerper, CD40 ligand antikoerper, TNF superfamily member 5 antikoerper, CD40LG antikoerper, cd40l antikoerper, Cd40lg antikoerper
- Hintergrund
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CD154, gp39, hCD40L, HIGM1, IGM, IMD3, T B cell activating molecule, T BAM, T-cell antigen Gp39, TNF-related activation protein, TrAP, Tumor necrosis factor ligand superfamily member 5 (TNFSF5),CD40 Ligand / CD154 / TRAP1 (Activation Marker of T-Lymphocytes)
Cellular localisation: Cell Surface and Secreted - Molekulargewicht
- 36kDa
- Gen-ID
- 959, 592244
- UniProt
- P29965
- Pathways
- NF-kappaB Signalweg, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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