Cyclin D1 Antikörper

Produktnummer ABIN6936590

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cyclin D1 in WB, FACS, IF und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Cyclin D1 Antikörper (ABIN6936590)

Target: Cyclin D1 (CCND1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin D1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: DCS-6

Produktdetails anti-Cyclin D1 Antikörper

Spezifität: Recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). About 40 % of breast carcinomas are positive for Cyclin D1. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Kreuzreaktivität (Details): Human. Monkey. Mouse. Rat.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Human full length recombinant cyclin D1 protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. Mantle cell lymphoma, breast or bladder carcinomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Cyclin D1

Target: Cyclin D1 (CCND1)

Andere Bezeichnung: CCND1

Hintergrund: B cell CLL/lymphoma 1, B cell leukemia 1, B-cell lymphoma 1 protein, BCL-1 oncogene, CCND1 protein, CCND1/FSTL3 fusion gene, CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene, CCND1/PTH fusion gene, cD1, Cyl 1, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1 oncogene,Cyclin D1 (G1-Cyclin & Mantle Cell Marker)
Cellular localisation: Nuclear

Molekulargewicht: 36kDa

Gen-ID: 595, 523852, 667996

UniProt: P24385

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, ER-Nucleus Signaling