Cyclin B2 Antikörper

Produktnummer ABIN6936587

Dieser Maus Monoklonal Antikörper detektiert spezifisch Cyclin B2 in ELISA, IHC und Coat. Es zeigt Reaktivität gegenüber Proben von Xenopus laevis.

Kurzübersicht für Cyclin B2 Antikörper (ABIN6936587)

Target: Cyclin B2 (CCNB2)

Reaktivität: Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin B2 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Coating (Coat)

Klon: X29-2

Produktdetails anti-Cyclin B2 Antikörper

Spezifität: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.

Kreuzreaktivität (Details): Mouse. Rat. Human. Xenopus laevis.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length Xenopus laevis Cyclin B2 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Human cervical tissue (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Cyclin B2

Target: Cyclin B2 (CCNB2)

Andere Bezeichnung: CCNB2

Hintergrund: Ccnb2, CycB2, Cyclin B2, G2 mitotic specific cyclin B2, HsT17299, MGC108931, MGC140694,Cyclin B2
Cellular localisation: Cytoplasm.

Molekulargewicht: 51kDa

Gen-ID: 9133, 194698

UniProt: O95067

Pathways: Zellzyklus, M Phase