Cyclin B2 Antikörper

Produktnummer ABIN6936587

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Cyclin B2 in ELISA, IHC und Coat. Er zeigt eine Reaktivität gegenüber Xenopus laevis.

Kurzübersicht für Cyclin B2 Antikörper (ABIN6936587)

Target: Cyclin B2 (CCNB2)

Reaktivität: Xenopus laevis

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin B2 Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Coating (Coat)

Klon: X29-2

Produktdetails anti-Cyclin B2 Antikörper

Spezifität: In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/ cyclin B enzyme, and tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The two B type cyclins, cyclin B1 and cyclin B2, have been shown to have distinct tissue distributions.

Kreuzreaktivität (Details): Mouse. Rat. Human. Xenopus laevis.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length Xenopus laevis Cyclin B2 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Human cervical tissue (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Cyclin B2

Target: Cyclin B2 (CCNB2)

Andere Bezeichnung: CCNB2

Hintergrund: Ccnb2, CycB2, Cyclin B2, G2 mitotic specific cyclin B2, HsT17299, MGC108931, MGC140694,Cyclin B2
Cellular localisation: Cytoplasm.

Molekulargewicht: 51kDa

Gen-ID: 9133, 194698

UniProt: O95067

Pathways: Zellzyklus, M Phase