Cyclin B1 Antikörper

Produktnummer ABIN6936584

Dieser Maus Monoklonal Antikörper erkennt spezifisch Cyclin B1 in IF, FACS, IP und BP. Er zeigt eine Reaktivität gegenüber Hamster.

Kurzübersicht für Cyclin B1 Antikörper (ABIN6936584)

Target: Cyclin B1 (CCNB1)

Reaktivität: Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Peptide (BP)

Klon: V92-1

Produktdetails anti-Cyclin B1 Antikörper

Spezifität: It recognizes a protein of 55-62 kDa, identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. So, cyclin B-p34cdc2 plays a critical role in G2 to M transition.

Kreuzreaktivität (Details): Human. Mouse. Hamster.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Hamster Cyclin B1 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Immunoprecipitation (1-2 μg/500 μg protein) (precipitates active CDK1/cyclin B1 complexes), Optimal dilution for a specific application should be determined.

Positive_Control: Cell line in logarithmic growth phase. Tonsil or testicular, endometrial, prostate or ovarian carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: CCNB1

Hintergrund: CCNB, CCNB1, CCNB1_HUMAN, G2 Mitotic Specific Cyclin B1,Cyclin B1 (G2- & M-phase Cyclin)
Cellular localisation: Nuclear

Molekulargewicht: 55-62kDa

Gen-ID: 891, 23960

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase