CAPNL1 Antikörper

Produktnummer ABIN6936578

Dieses Maus Monoklonal-Antikörper erkennt spezifisch CAPNL1 in ELISA, Coat und IHC (f). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CAPNL1 Antikörper (ABIN6936578)

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CAPNL1 Antikörper ist unkonjugiert

Applikation: ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: CAPN1-1530

Produktdetails anti-CAPNL1 Antikörper

Spezifität: Calpain 1 is an intracellular calcium-dependent protease that cleaves cytoskeletal and sub-membranous proteins. Calpains are non-lysosomal, calcium-activated intracellular cysteine proteases. Calpains mediate specific Ca2+- dependent processes including cell fusion, mitosis and meiosis. Calpains are heterodimers of a small regulatory subunit and one of three large catalytic subunits, designated Calpain 1, Calpain 2 and Calpain p94. Calpastatin regulates Calpain by inhibiting both the proteolytic activity of Calpain and its binding to membranes. Calpastatin exists in two types, tissue type and erythrocyte type, resulting from both alternative splicing and proteolytic processing. Calpain 1 co-localizes with human leukocyte antigen-DR (HLA-DR) on activated microglia in the aging brain. Calpain influences the process of spermatogenesis and the events preceding fertilization, such as the acrosome reaction.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human CAPN1 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.

Positive_Control: HeLa cells. Skin or Tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu CAPNL1

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Andere Bezeichnung: CAPN1

Hintergrund: Calcium dependent protease small subunit 1, Calcium-activated neutral proteinase 1, Calpain 1 large subunit, Calpain mu-type, Calpain regulatory subunit, Calpain small subunit 1, Calpain, large polypeptide L1, Calpain-1 catalytic subunit, CANP small subunit, CANP1, CANPL1, CAPN1, Cell proliferation-inducing gene 30 protein, Micromolar-calpain, Mu Calpain,Calpain 1
Cellular localisation: Cytoplasm and Cell Surface

Molekulargewicht: 80kDa

Gen-ID: 823, 502842

UniProt: P07384

Pathways: Apoptose