Calnexin Antikörper (AA 1-300)
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- Target Alle Calnexin (CANX) Antikörper anzeigen
- Calnexin (CANX)
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Bindungsspezifität
- AA 1-300
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Calnexin Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Coating (Coat), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Spezifität
- It recognizes a protein of 90 kDa, which is identified as Calnexin. Secretory and transmembrane proteins are synthesized on polysomes and translocate into the endoplasmic reticulum (ER) where they are often modified by the formation of disulfide bonds, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including calnexin. It is a calcium-binding, endoplasmic reticulum (ER)-associated protein that interacts transiently with newly synthesized N-linked glycoproteins, facilitating protein folding and assembly. It may also play a central role in the quality control of protein folding by retaining incorrectly folded protein subunits within the ER for degradation.
- Keine Kreuzreaktivität
- Ratte (Rattus)
- Kreuzreaktivität (Details)
- Human. Does not react with Rat.
- Aufreinigung
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant N-terminal fragment of human Calnexin protein (around aa 1-300) (exact sequence is proprietary)
- Klon
- CANX-1541
- Isotyp
- IgG2b kappa
- Top Product
- Discover our top product CANX Primärantikörper
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- Applikationshinweise
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Known_Application: ELISA (Use Ab at 2-4 μg/mL for coating) (Order Ab without BSA), Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa, MCF-7 or U2OS cells. Kidney or small intestine.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Konservierungsmittel
- Azide free
- Lagerung
- -20 °C,-80 °C
- Informationen zur Lagerung
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Haltbarkeit
- 24 months
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- Target
- Calnexin (CANX)
- Andere Bezeichnung
- CANX (CANX Produkte)
- Synonyme
- CNX antikoerper, IP90 antikoerper, P90 antikoerper, 1110069N15Rik antikoerper, AI988026 antikoerper, Cnx antikoerper, D11Ertd153e antikoerper, canx antikoerper, clgn antikoerper, clnx antikoerper, cnx antikoerper, PP90 antikoerper, zgc:63524 antikoerper, wu:fe06b12 antikoerper, CANX antikoerper, calnexin antikoerper, CalNeXin antikoerper, calnexin L homeolog antikoerper, CANX antikoerper, Canx antikoerper, cnx-1 antikoerper, canx.L antikoerper, canx antikoerper, LOC100283947 antikoerper
- Hintergrund
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Calnexin, CANX, CNX, IP90, Major histocompatibility complex class I antigen-binding protein p88, P90,Calnexin (Endoplasmic Reticulum Marker)
Cellular localisation: Cytoplasmic - Molekulargewicht
- 90kDa
- Gen-ID
- 821, 567968
- UniProt
- P27824
- Pathways
- MAPK Signalweg, Thyroid Hormone Synthesis
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