APC Antikörper (AA 1-433)

Produktnummer ABIN6936423

Der Maus Monoklonal Anti-APC-Antikörper wurde für WB, ELISA, IF, FACS und Coat validiert. Er ist geeignet, APC in Proben von Human zu detektieren.

Kurzübersicht für APC Antikörper (AA 1-433) (ABIN6936423)

Target: APC (Adenomatous Polyposis Coli (APC))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser APC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Coating (Coat)

Klon: ALi 12-28

Produktdetails anti-APC Antikörper

Bindungsspezifität: AA 1-433

Spezifität: The adenomatous polyposis syndromes, familial adenomatous polyposis (FAP) and Gardner's syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. The cloning of the APC gene revealed a ubiquitously expressed protein, 2,843 amino acids in length, which is frequently mutated in patients suffering from FAP and GS. APC has been found to be associated with structural components of intracellular junctions. Functions as a tumor suppressor, promoting rapid degradation of CTNNB1 and is a negative regulator of Wnt signaling. Also plays a role in HGF-induced cell migration. Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex. It is required for the localization of MACF1 to the cell membrane and this localization of MACF1 is critical for its function in microtubule stabilization.

Keine Kreuzreaktivität: Maus

Kreuzreaktivität (Details): Human. Does not react with Mouse.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human APC protein (around aa 1-433)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: ELISA (For coating, order antibody without BSA), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: COLO 320DM whole cell lysate. HCT116 cells. Tonsil, placenta, breast or colon.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu APC

Target: APC (Adenomatous Polyposis Coli (APC))

Andere Bezeichnung: APC

Hintergrund: Adenomatous Polyposis Coli, CC1, Deleted in polyposis 2.5 (DP2.5), DP2, DP3, FAP, FPC, GS, Protein APC,APC / Adenomatous Polyposis Coli / FAP (Tumor Suppressor)
Cellular localisation: Cell membrane and cytoplasm.

Molekulargewicht: 66kDa

Gen-ID: 324, 158932

UniProt: P25054

Pathways: WNT Signalweg, Stem Cell Maintenance, Cell-Cell Junction Organization