PLAP Antikörper

Produktnummer ABIN6936399

Produktdetails anti-PLAP Antikörper

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PLAP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Spezifität: Reacts with a 70 kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Kreuzreaktivität (Details): Human.

Aufreinigung: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant human PLAP protein

Klon: GM022

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise:

Known_Application: Western Blot (1-2 μg/mL), Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT)(No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.)Optimal dilution for a specific application should be determined.

Positive_Control: JEG-3 or HepG2 cells. Placenta or seminoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Konservierungsmittel: Azide free

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Haltbarkeit: 24 months

Details zu PLAP

Target: PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))

Andere Bezeichnung: ALPP (ALPP Produkte)

Synonyme: ECK0378 antikoerper, JW0374 antikoerper, psiA antikoerper, ALP antikoerper, PALP antikoerper, PLAP antikoerper, PLAP-1 antikoerper, ILC antikoerper, CTACK antikoerper, ESkine antikoerper, Akp2 antikoerper, alp antikoerper, zgc:56672 antikoerper, DDBDRAFT_0205491 antikoerper, DDBDRAFT_0231570 antikoerper, DDB_0205491 antikoerper, DDB_0231570 antikoerper, ALPI antikoerper, Actn2lp antikoerper, Alp antikoerper, bacterial alkaline phosphatase antikoerper, alkaline phosphatase, placental antikoerper, alkaline phosphatase, placental type antikoerper, C-C motif chemokine ligand 27 antikoerper, alkaline phosphatase, liver/bone/kidney antikoerper, alkaline phosphatase antikoerper, alkaline phosphatase, intestinal antikoerper, PDZ and LIM domain 3 antikoerper, phoA antikoerper, ALPP antikoerper, LOC100436725 antikoerper, CCL27 antikoerper, alpl antikoerper, alp antikoerper, ALPI antikoerper, Pdlim3 antikoerper, Alpp antikoerper

Hintergrund: Alkaline phosphatase placental type, Alkaline phosphatase Regan Isozyme, ALP, Alp1, ALPP, Germ-cell alkaline phosphatase, nagao Isozyme, PALP, Placental alkaline phosphatase 1, placental heat-stable alkaline phosphatase, PLAP-1, PLAP1,Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker)
Cellular localisation: Cytoplasmic and cell surface

Molekulargewicht: 70kDa

Gen-ID: 250, 284255

UniProt: P05187