CDKN1B Antikörper (CF®488A)

Produktnummer ABIN6935028

Der Maus Monoklonal Anti-CDKN1B-Antikörper wurde für IF, FACS und T validiert. Er ist geeignet, CDKN1B in Proben von Human zu detektieren.

Kurzübersicht für CDKN1B Antikörper (CF®488A) (ABIN6935028)

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDKN1B Antikörper ist konjugiert mit CF®488A

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Test (T)

Klon: SX53G8

Produktdetails anti-CDKN1B Antikörper (CF®488A)

Spezifität: This MAb recognizes a 27 kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Kreuzreaktivität (Details): Human, Mouse, Rat, Monkey,

Aufreinigung: Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules.

Immunogen: Purified GST-p27 fusion protein of human origin

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Positive Control: ZR75, T47D, SK-BR-3, MDA-MB-231, MCF7 cells. Tonsil, Breast or Colon Ca.

Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100),Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 100 μg/mL

Buffer: Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8 °C. Antibody is stable for 24 months. Non-hazardous. Also available WITHOUT BSA & azide at 1.0mg/ml.

Haltbarkeit: 24 months

Details zu CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Andere Bezeichnung: CDKN1B

Hintergrund: CDKN1B, CDKN4, Cyclin Dependent Kinase Inhibitor 1B, Cyclin-dependent kinase inhibitor p27 Kip1, KIP1, MEN1B, MEN4,p27Kip1 (Mitotic Inhibitor/Suppressor Protein)
Cellular localisation: Nuclear

Molekulargewicht: 25-26kDa

Gen-ID: 1027, 238990

UniProt: P46527

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagie