Cyclin D1 Antikörper (PE)

Produktnummer ABIN6934200

Der Maus Monoklonal Anti-Cyclin D1-Antikörper wurde für FACS, IF und T validiert. Er ist geeignet, Cyclin D1 in Proben von Human, Maus, Ratte und Affe zu detektieren.

Kurzübersicht für Cyclin D1 Antikörper (PE) (ABIN6934200)

Target: Cyclin D1 (CCND1)

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin D1 Antikörper ist konjugiert mit PE

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Test (T)

Klon: DCS-6

Produktdetails anti-Cyclin D1 Antikörper (PE)

Spezifität: Recognizes a protein of 36 kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Aufreinigung: Purified by Protein A/G

Immunogen: Human recombinant full length cyclin D1 protein

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. ~60 % of mantle cell lymphomas and ~40 % of breast carcinomas are positive.

Known Application: Flow Cytometry (5 µL per test per one million cells or 5 µL per 100 µL of whole blood),Immunofluorescence (1:50-1:100 for 30 minutes at RT)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 100 μg/mL

Buffer: 10 mM PBS with 0.05 % BSA and 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 4 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu Cyclin D1

Target: Cyclin D1 (CCND1)

Andere Bezeichnung: CCND1

Molekulargewicht: 36kDa

Gen-ID: 595

UniProt: P24385

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, ER-Nucleus Signaling