EPAS1 Antikörper (AA 1-130)

Produktnummer ABIN686662

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch EPAS1 in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) und IHC (fro). Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Affe und Schaf und wurde in 1 Publikation erwähnt.

Kurzübersicht für EPAS1 Antikörper (AA 1-130) (ABIN686662)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reaktivität: Human, Ratte, Maus, Affe, Schaf

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EPAS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-EPAS1 Antikörper

Bindungsspezifität: AA 1-130

Kreuzreaktivität: Human, Affe, Maus, Ratte, Schaf

Homologie: Cow,Pig,Horse

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse HIF2 Alpha

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-EPAS1 Antikörper (ABIN686662)

Li, Chen, Shao, Tang, Chen, Chen, Xu: "Oxidative stress induces the decline of brain EPO expression in aging rats." in: Experimental gerontology, Vol. 83, pp. 89-93, (2016) (PubMed).

Details zu EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Andere Bezeichnung: HIF2 alpha

Hintergrund:

Synonyms: HLF, HRF, MOP2, HIF2A, bHLHe73, HIF-2alpha, Endothelial PAS domain-containing protein 1, EPAS-1, HIF-1-alpha-like factor, mHLF, HIF-related factor, Hypoxia-inducible factor 2-alpha, HIF-2-alpha, HIF2-alpha, Epas1

Background: Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBPB and probably EP3. Interaction with redox regulatory protein APEX seems to activate CTAD (By similarity).

Gen-ID: 13819

UniProt: P97481

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effekt