LDLR Antikörper (AA 184-196)

Produktnummer ABIN233315

Der Kaninchen Polyklonal Anti-LDLR-Antikörper wurde für WB, IHC, ELISA, FACS und IHC (fro) validiert. Er ist geeignet, LDLR in Proben von Human und Ratte zu detektieren.

Kurzübersicht für LDLR Antikörper (AA 184-196) (ABIN233315)

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LDLR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-LDLR Antikörper

Bindungsspezifität: AA 184-196

Spezifität: Recognizes human LDLR. Does not inhibit binding of LDL. In Western Blot, recognizes the 160kD of LDLR and a 120kD band of the LDLR precursor from fibroblasts, hepatocytes and monocytic cells cultured in the presence of lipoprotein-deficient serum. Sequence not conserved in VLVL Receptor and LRP. Species cross-reactivity: rat. Species sequence Homology: mouse and bovine.

Aufreinigung: Protein A purified

Immunogen: Synthetic peptide of the complement homology region within the ligand binding site of the LDL receptor extracellular domain. Epitope between aa184-196, the linker region between repeats 4 and 5.

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Flo (1:5), IHC, IHC-Fr (1:10), WB (1:50 - 1:100)

Usage: Suitable for use in ELISA, Western Blot, FACS and Immunohistochemistry. Western Blot: 1:50-1:100 using ECL (non-reducing). FACS: 1:5. Immunohistochemistry: 1:10. Note: It is best to work with partially purified material. In the case of rat (probably also mouse), induce LDLR expression prior to the isolation of culture cells or tissue homogenate. It is always better to use cultured cells. In rat, use estradiol to induce LDLR expression. Positive control: HepG2 cells and liver sections.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.5 % BSA.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu LDLR

Target: LDLR (Low Density Lipoprotein Receptor (LDLR))

Andere Bezeichnung: LDLR / LDL Receptor

Hintergrund: Name/Gene ID: LDLR

Synonyms: LDLR, FHC, LDL receptor, LDLCQ2

Gen-ID: 3949

UniProt: P01130

Pathways: Hepatitis C, Lipid Metabolism