Hexokinase 2 Antikörper (AA 74-123)

Produktnummer ABIN462257

Der Kaninchen Polyklonal Anti-Hexokinase 2-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, Hexokinase 2 in Proben von Human, Maus, Ratte, Hund, Kaninchen, Schwein, Rind (Kuh), Pferd, Affe, Meerschweinchen, Zebrafisch (Danio rerio), Hamster und Fledermaus zu detektieren.

Kurzübersicht für Hexokinase 2 Antikörper (AA 74-123) (ABIN462257)

Target: Hexokinase 2 (HK2)

Reaktivität: Human, Maus, Ratte, Hund, Kaninchen, Schwein, Rind (Kuh), Pferd, Affe, Meerschweinchen, Zebrafisch (Danio rerio), Hamster, Fledermaus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Hexokinase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-Hexokinase 2 Antikörper

Bindungsspezifität: AA 74-123

Marke: IHC-plus™

Spezifität: Human HK2 / Hexokinase 2

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig (100%) Chicken, Zebrafish (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa74-123 of human HK2 (P52789, NP_000180). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Guinea pig (100%), Sablefish, Stickleback (92%), Orangutan, Opossum, Turkey, Zebra finch, Chicken, Platypus, Xenopus, Zebrafish, Sea squirt (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:12500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu Hexokinase 2

Target: Hexokinase 2 (HK2)

Andere Bezeichnung: HK2 / Hexokinase 2

Hintergrund: Name/Gene ID: HK2
Subfamily: Hexokinase
Family: Non-protein Kinase

Synonyms: HK2, Hexokinase II, Hexokinase type II, Hexokinase-2, muscle, HK II, HXK2, Hexokinase-2, Muscle form hexokinase, Hexokinase 2, HKII, Hxk 2

Gen-ID: 3099

NCBI Accession: NP_000180

UniProt: P52789

Pathways: PI3K-Akt Signalweg, Carbohydrate Homeostasis, Warburg Effekt