LDHB Antikörper (C-Term)

Produktnummer ABIN6744435

Der Kaninchen Polyklonal Anti-LDHB-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, LDHB in Proben von Human, Affe, Hund, Kaninchen und Huhn zu detektieren.

Kurzübersicht für LDHB Antikörper (C-Term) (ABIN6744435)

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Reaktivität: Human, Affe, Hund, Kaninchen, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LDHB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-LDHB Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human LDHB

Homologie: Percent identity by BLAST analysis: Human, Dog, Rabbit, Chicken (100%) Mouse, Rat, Bovine, Horse, Pig (92%) Zebrafish (90%).

Aufreinigung: Affinity purified

Immunogen: Synthetic peptide from C-Terminus of human LDHB (P07195, NP_002291). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Ferret, Dolphin, Elephant, Panda, Dog, Rabbit, Opossum, Turkey, Chicken (100%), Galago, Mouse, Rat, Hamster, Bovine, Horse, Pig, Guinea pig (92%), Catfish (91%), Zebrafish (90%), Zebra finch (85%), Salmon (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu LDHB

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Andere Bezeichnung: LDHB / LDH-B

Hintergrund: Name/Gene ID: LDHB

Synonyms: LDHB, LDH heart subunit, Lactate dehydrogenase H chain, Lactate dehydrogenase B, LDH-B, TRG-5, LDH-H, LDHBD

Gen-ID: 3945

NCBI Accession: NP_002291

UniProt: P07195

Pathways: Warburg Effekt