IDH1 Antikörper (AA 290-339)

Produktnummer ABIN6741619

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch IDH1 in WB, IHC und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Hund, Affe, Pferd, Meerschweinchen, Rind (Kuh), Kaninchen, Schaf, Schwein, Hamster, Huhn und Xenopus laevis.

Kurzübersicht für IDH1 Antikörper (AA 290-339) (ABIN6741619)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reaktivität: Human, Maus, Ratte, Hund, Affe, Pferd, Meerschweinchen, Rind (Kuh), Kaninchen, Schaf, Schwein, Hamster, Huhn, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IDH1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-IDH1 Antikörper

Bindungsspezifität: AA 290-339

Marke: IHC-plus™

Spezifität: Human IDH1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken, Xenopus (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa290-339 of human IDH1 (Q8LFC0, NP_005887). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Hamster, Dog, Cat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Beetle (100%), Panda, Armadillo, Rice, Arabidopsis (92%), Drosophila (85%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu IDH1

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Andere Bezeichnung: IDH1 / IDH

Hintergrund: Name/Gene ID: IDH1

Synonyms: IDH1, IDCD, IDP, IDPC, PICD, NADP(+)-specific ICDH, IDH, Oxalosuccinate decarboxylase

Gen-ID: 3417

NCBI Accession: NP_005887

UniProt: O75874

Pathways: Warburg Effekt