HOXA5 Antikörper (C-Term)

Produktnummer ABIN6741580

Der Kaninchen Polyklonal Anti-HOXA5-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, HOXA5 in Proben von Human, Ratte, Maus, Schwein, Kaninchen, Rind (Kuh), Hund, Pferd, Meerschweinchen, Zebrafisch (Danio rerio), Drosophila melanogaster, Fledermaus, Affe, Huhn, Hamster und Xenopus laevis zu detektieren.

Kurzübersicht für HOXA5 Antikörper (C-Term) (ABIN6741580)

Target: HOXA5 (Homeobox A5 (HOXA5))

Reaktivität: Human, Ratte, Maus, Schwein, Kaninchen, Rind (Kuh), Hund, Pferd, Meerschweinchen, Zebrafisch (Danio rerio), Drosophila melanogaster, Fledermaus, Affe, Huhn, Hamster, Xenopus laevis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HOXA5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HOXA5 Antikörper

Bindungsspezifität: C-Term

Marke: IHC-plus™

Spezifität: Human HOXA5

Homologie: Percent identity by BLAST analysis: Human, Mouse, Dog, Pig, Guinea pig, Zebrafish (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human HOXA5 (A2D5Y4, NP_061975). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Baboon, Monkey, Galago, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Armadillo, Platypus, Lizard, Xenopus, Trout, Seabass, Salmon, Stickleback, Medaka, Pufferfish, Zebrafish, Drosophila, Water flea, Beetle (100%), Smelt (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 - 10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled Water.

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu HOXA5

Target: HOXA5 (Homeobox A5 (HOXA5))

Andere Bezeichnung: HOXA5

Hintergrund: Name/Gene ID: HOXA5

Synonyms: HOXA5, Homeo box A5, Homeobox A5, Homeo box 1C, Homeobox protein Hox-1C, HOX1, Homeobox protein Hox-A5, Homeobox protein HOXA5, HOX1.3, HOX1C

Gen-ID: 3202

NCBI Accession: NP_061975

UniProt: P20719