ATF4 Antikörper (N-Term)

Produktnummer ABIN462203

Der Kaninchen Polyklonal Anti-ATF4-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, ATF4 in Proben von Human und Maus zu detektieren.

Kurzübersicht für ATF4 Antikörper (N-Term) (ABIN462203)

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-ATF4 Antikörper

Bindungsspezifität: N-Term

Marke: IHC-plus™

Spezifität: Mouse ATF4

Homologie: Percent identity by BLAST analysis: Mouse (100%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of mouse ATF4 (Q61328, NP_033846). Percent identity by BLAST analysis: Mouse (100%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Andere Bezeichnung: ATF4

Hintergrund: Name/Gene ID: ATF4
Family: Transcription factor

Synonyms: ATF4, CREB-2, CREB2, TXREB, DNA-binding protein TAXREB67, TAXREB67

Gen-ID: 468

NCBI Accession: NP_033846

UniProt: P18848

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response