EN1 Antikörper (AA 252-301)
Quick Overview for EN1 Antikörper (AA 252-301) (ABIN6741515)
Target
Alle EN1 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Bindungsspezifität
- AA 252-301
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Spezifität
- Human EN1
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Homologie
- Percent identity by BLAST analysis: Pig, Guinea pig (93%) Xenopus (92%) Rabbit (86%).
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Aufreinigung
- Immunoaffinity purified
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Immunogen
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Synthetic peptide located between aa252-301 of human EN1 (Q05925, NP_001417). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Mouse, Rat, Bovine, Pig, Guinea pig, Chicken, Platypus, Lizard (100%), Gibbon, Galago, Elephant, Bat, Horse, Opossum (93%), Rabbit, Xenopus, Zebrafish (92%).
Type of Immunogen: Synthetic peptide -
Isotyp
- IgG
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Applikationshinweise
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Approved: WB (0.2 - 1 μg/mL)
Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500. -
Kommentare
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Target Species of Antibody: Human
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Distilled water
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Konzentration
- Lot specific
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Buffer
- Lyophilized from PBS with 2 % sucrose
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Handhabung
- Avoid repeat freeze-thaw cycles.
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- EN1 (Engrailed Homeobox 1 (EN1))
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Andere Bezeichnung
- EN1 / Engrailed
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Hintergrund
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Name/Gene ID: EN1
Synonyms: EN1, Engrailed 1, Engrailed homolog 1, Homeobox protein en-1, Homeobox protein engrailed-1, Hu-En-1, Engrailed homeobox 1 -
Gen-ID
- 2019
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NCBI Accession
- NP_001417
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UniProt
- Q05925
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Pathways
- Dopaminergic Neurogenesis
Target
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