TCF4 Antikörper (AA 73-122)

Produktnummer ABIN6736759

Der Kaninchen Polyklonal Anti-TCF4-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, TCF4 in Proben von Human, Maus, Ratte, Pferd, Schwein, Hund, Affe, Rind (Kuh) und Kaninchen zu detektieren.

Kurzübersicht für TCF4 Antikörper (AA 73-122) (ABIN6736759)

Target: TCF4 (Transcription Factor 4 (TCF4))

Reaktivität: Human, Maus, Ratte, Pferd, Schwein, Hund, Affe, Rind (Kuh), Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TCF4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-TCF4 Antikörper

Bindungsspezifität: AA 73-122

Marke: IHC-plus™

Spezifität: Human TCF4

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Horse, Pig (100%) Guinea pig (92%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa73-122 of human TCF4 (P15884, NP_003190). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum (100%), Guinea pig (92%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from 2 % Sucrose, PBS.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu TCF4

Target: TCF4 (Transcription Factor 4 (TCF4))

Andere Bezeichnung: TCF4

Hintergrund: Name/Gene ID: TCF4
Family: Transcription factor

Synonyms: TCF4, BHLHb19, E2-2, ITF-2, ITF2, PTHS, SEF2-1B, SEF2-1, SEF2-1A, SL3-3 enhancer factor 2, SEF2, SEF-2, TCF-4, Transcription factor 4

Gen-ID: 6925

NCBI Accession: NP_003190

UniProt: P15884

Pathways: WNT Signalweg, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus