FOXA1 Antikörper (AA 395-444)

Produktnummer ABIN6736377

Der Kaninchen Polyklonal Anti-FOXA1-Antikörper wurde für WB, IHC und IHC (p) validiert. Er ist geeignet, FOXA1 in Proben von Human, Ratte, Maus, Hund, Rind (Kuh), Schwein, Zebrafisch (Danio rerio), Meerschweinchen und Affe zu detektieren.

Kurzübersicht für FOXA1 Antikörper (AA 395-444) (ABIN6736377)

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Reaktivität: Human, Ratte, Maus, Hund, Rind (Kuh), Schwein, Zebrafisch (Danio rerio), Meerschweinchen, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FOXA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-FOXA1 Antikörper

Bindungsspezifität: AA 395-444

Marke: IHC-plus™

Spezifität: Human FOXA1

Homologie: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig (100%) Zebrafish (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa395-444 of human FOXA1 (NP_004487). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Galago, Mouse, Rat, Elephant, Panda, Dog, Bovine, Pig, Opossum, Guinea pig, Lizard (100%), Xenopus (93%), Bat (92%), Stickleback, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu FOXA1

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Andere Bezeichnung: FOXA1

Hintergrund: Name/Gene ID: FOXA1
Family: Transcription factor

Synonyms: FOXA1, Forkhead box A1, Forkhead box protein A1, HNF-3-alpha, HNF-3A, TCF-3A, TCF3A, HNF3A, Transcription factor 3A

Gen-ID: 3169

NCBI Accession: NP_004487

UniProt: P55317

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis