HCN1 Antikörper (AA 346-368)

Produktnummer ABIN6735304

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch HCN1 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus und Kaninchen.

Kurzübersicht für HCN1 Antikörper (AA 346-368) (ABIN6735304)

Target: HCN1 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1))

Reaktivität: Human, Ratte, Maus, Kaninchen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HCN1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-HCN1 Antikörper

Bindungsspezifität: AA 346-368

Spezifität: The pore region of HCN1 (HCNP15-P) is very conserved among various HCNs. HCN1: mouse, rat, human, rabbit=100 % , HCN2: mouse, rat, human (86 %), HCN3: mouse (95 %), rat (90 %), HCN4: rat, human (95 %). The HCNP15-P sequence is also conserved in insect (Helothis virescens, 78 %), Drosophila (78 %) and Sea urchin (S. purpuratus, 69 %) HCNs. Therefore, antibodies to HCNP15-P should react with most HCNs and related sequences. Antibody reactivity in various species is not known. The HCNP15-P control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 μg control peptide per 1 μg of aff pure IgG or 1 μL antiserum) to confirm antibody specificity. This antibody may be useful in studying various HCNs and to examine the possibility of immunoblocking the pore region and impact on the functionality of HCNs proteins.

Aufreinigung: Immunoaffinity purified

Immunogen: A 23-aa peptide sequence corresponding to the pore region of human HCN1 corresponding to amino acids 346-368.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:10000 - 1:100000), WB (1 - 10 μg/mL)

Usage: Suitable for use in ELISA, Western Blot. Western Blot: 1:1000-1:5000 for neat serum and 1-10 μg/mL for affinity pure using ECL. ELISA: 1:10000-1:100000 using 50-100 ng of control peptide/well.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % BSA.

Handhabung: avoid freeze thaw cycles. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.

Details zu HCN1

Target: HCN1 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1))

Andere Bezeichnung: HCN1

Hintergrund: Name/Gene ID: HCN1
Subfamily: Potassium channel - cyclic nucleotide gated
Family: Ion Channel

Synonyms: HCN1, BCNG1, BCNG-1, HAC2, HAC-2

Gen-ID: 348980

UniProt: O60741

Pathways: Asymmetric Protein Localization