GST Antikörper (DyLight 800)

Produktnummer ABIN6720739

Hersteller Produkt- Nr.: 600-145-200

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch GST in WB, FLISA und FM. Er zeigt eine Reaktivität gegenüber Schistosoma japonicum und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für GST Antikörper (DyLight 800) (ABIN6720739)

Target: GST (Glutathione S Transferase (GST))

Reaktivität: Schistosoma japonicum

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GST Antikörper ist konjugiert mit DyLight 800

Applikation: Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)

Produktdetails anti-GST Antikörper (DyLight 800)

Hersteller Produkt- Nr.: 600-145-200

Hersteller: Rockland

Verwendungszweck: GST Antibody Dylight™ 800 Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Glutathione-S-Transferase (GST).

Aufreinigung: This product was prepared from monospecific antiserum by immunoaffinity chromatography using GST (Schistosoma japonicum) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: The immunogen is full length GST isolated from Schistosoma japonicum.

Isotyp: IgG

Labeling Ratio: 2.0

Anwendungsinformationen

Applikationshinweise:

FLISA_Dilution: >1:20,000

IF_Microscopy_Dilution: >1:5,000

Western_Blot_Dilution: 1:10,000 - 1:25,000

Other: User Optimized

Kommentare:

Suggested Applications: ELISA, WB
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-GST Antikörper (DyLight 800) (ABIN6720739)

Ng, Machida: "Rosette Assay: Highly Customizable Dot-Blot for SH2 Domain Screening." in: Methods in molecular biology (Clifton, N.J.), Vol. 1555, pp. 437-451, (2018) (PubMed).

Miller, Yan, Machida, Kiraly, Levy, Wu, Lam, Abbott, Koleske, Eipper, Mains: "Brain Region and Isoform-Specific Phosphorylation Alters Kalirin SH2 Domain Interaction Sites and Calpain Sensitivity." in: ACS chemical neuroscience, Vol. 8, Issue 7, pp. 1554-1569, (2018) (PubMed).

Details zu GST

Target: GST (Glutathione S Transferase (GST))

Andere Bezeichnung: GST

Hintergrund:

Synonyms: goat anti-GST antibody DyLight™ 800 conjugation, DyLight™ 800 conjugated goat anti-GST antibody, Glutathione-S-Transferase, GST antibody, anti-GST antibody, anti-Glutathione-S-Transferase antibody

Background: Rockland produces a wide range of GST antibodies in our laboratories. Select GST antibodies from several monoclonal and/or polyclonal GST antibodies listed below. Select appropriate GST antibodies for your research by isotype, epitope, applications and species reactivity. GST (Glutathione-S-Transferase) is a protein expression tag commonly used in molecular biology. Anti-GST will react with synthetic construct present in most known GST containing cloning or expression vectors. GST is responsible for the conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. The amino acid sequence GST is highly conserved in most organisms including mammals. GST exists as a 26 kDa homodimer.